![]() ![]() ![]() Beads were washed, and low-pH elution was performed. The complex was then incubated with Thermo Scientific Pierce Protein A/G Magnetic Beads for 1 hr at room temperature. ![]() Lysate containing 500 μg of protein was then incubated with 5 μg of phospho-tyrosine antibody overnight at 4✬. Cell lysates were prepared in Thermo Scientific Pierce IP Lysis Buffer with Thermo Scientific Protease and Phosphatase Inhibitor Mini Tablets, EDTA-Free, or with no inhibitor. (A) HCT116 cells were serum-starved, then either treated with EGF for 15 min or left as control cells. Protein phosphorylation in cell extracts is broadly preserved by Thermo Scientific Phosphatase Inhibitor Mini Tablets. The ExpiSf Expression System is the first-ever chemically defined baculovirus-insect cell protein expression system, delivering superior yields (3x more protein) and consistent performance run after run using a fast, streamlined workflow. The Gibco Expi293 Expression System is a completely optimized system consisting of Expi293F(TM) cells that have been adapted to high-density, serum-free suspension culture in Expi293(TM) Expression Medium, along with specially designed transfection reagents and enhancers, that enable recombinant 293-derived recombinant protein expression in just five to seven days, with a 2-10-fold increase in protein yields compared to previous generation transient expression systems That means you can start your research work in CHO cells and stay in CHO cells through discovery. The Gibco ExpiCHO Expression System is a completely optimized system consisting of ExpiCHO-S(TM) cells that have been adapted to high-density, serum-free suspension culture in ExpiCHO(TM) Expression Medium, along with specially designed transfection reagents and enhancers, that provide the highest yields possible in a transient system (up to 3g/L). ![]()
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